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mouse anti paired box protein 7  (Developmental Studies Hybridoma Bank)


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    Developmental Studies Hybridoma Bank mouse anti paired box protein 7
    Mouse Anti Paired Box Protein 7, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 99/100, based on 2479 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti paired box protein 7/product/Developmental Studies Hybridoma Bank
    Average 99 stars, based on 2479 article reviews
    mouse anti paired box protein 7 - by Bioz Stars, 2026-06
    99/100 stars

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    Santa Cruz Biotechnology anti mouse paired box protein pax7 monoclonal antibody
    Identification of SCs, construction of the expression vector and the effect of two haplotypes on MEG3 transcription. (A) SCs were identified by immunolabeling with <t>anti-Pax7</t> (red) monolyclonal antibody; Nuclear are counterstained with DAPI (blue); Merged images represented overlays of <t>Pax7</t> (red) and nuclear staining by DAPI (blue). (B) A FLAG tag sequence (pink) was added at the 3′ end of the MEG3 sequence (gray). This fragment was inserted into the pcDNA3.1(+) vector between the EcoR I and Xho I site. (C) The RT-PCR results of SCs transfected with pcDNA3.1-MEG3-CCCA, pcDNA3.1-MEG3-TTCC and pcDNA 3.1 (+) vector for 48 h. M: Marker 2000, Line 1–3: the RT-PCR results of pcDNA3.1-MEG3-CCCA group, pcDNA3.1-MEG3-TTCC group and pcDNA 3.1(+) group were amplified by MEG3-FLAG, respectively, Line 4: the RT-PCR results of pcDNA 3.1(+) group were amplified by Primer-GAPDH. (D) The effect of two haplotypes on MEG3 transcription. The significance test: ∗∗ P < 0.01.
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    Developmental Studies Hybridoma Bank antibodies mouse anti paired box protein 7
    Identification of SCs, construction of the expression vector and the effect of two haplotypes on MEG3 transcription. (A) SCs were identified by immunolabeling with <t>anti-Pax7</t> (red) monolyclonal antibody; Nuclear are counterstained with DAPI (blue); Merged images represented overlays of <t>Pax7</t> (red) and nuclear staining by DAPI (blue). (B) A FLAG tag sequence (pink) was added at the 3′ end of the MEG3 sequence (gray). This fragment was inserted into the pcDNA3.1(+) vector between the EcoR I and Xho I site. (C) The RT-PCR results of SCs transfected with pcDNA3.1-MEG3-CCCA, pcDNA3.1-MEG3-TTCC and pcDNA 3.1 (+) vector for 48 h. M: Marker 2000, Line 1–3: the RT-PCR results of pcDNA3.1-MEG3-CCCA group, pcDNA3.1-MEG3-TTCC group and pcDNA 3.1(+) group were amplified by MEG3-FLAG, respectively, Line 4: the RT-PCR results of pcDNA 3.1(+) group were amplified by Primer-GAPDH. (D) The effect of two haplotypes on MEG3 transcription. The significance test: ∗∗ P < 0.01.
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    Identification of SCs, construction of the expression vector and the effect of two haplotypes on MEG3 transcription. (A) SCs were identified by immunolabeling with anti-Pax7 (red) monolyclonal antibody; Nuclear are counterstained with DAPI (blue); Merged images represented overlays of Pax7 (red) and nuclear staining by DAPI (blue). (B) A FLAG tag sequence (pink) was added at the 3′ end of the MEG3 sequence (gray). This fragment was inserted into the pcDNA3.1(+) vector between the EcoR I and Xho I site. (C) The RT-PCR results of SCs transfected with pcDNA3.1-MEG3-CCCA, pcDNA3.1-MEG3-TTCC and pcDNA 3.1 (+) vector for 48 h. M: Marker 2000, Line 1–3: the RT-PCR results of pcDNA3.1-MEG3-CCCA group, pcDNA3.1-MEG3-TTCC group and pcDNA 3.1(+) group were amplified by MEG3-FLAG, respectively, Line 4: the RT-PCR results of pcDNA 3.1(+) group were amplified by Primer-GAPDH. (D) The effect of two haplotypes on MEG3 transcription. The significance test: ∗∗ P < 0.01.

    Journal: Frontiers in Genetics

    Article Title: Effects and Molecular Mechanism of Single-Nucleotide Polymorphisms of MEG3 on Porcine Skeletal Muscle Development

    doi: 10.3389/fgene.2021.607910

    Figure Lengend Snippet: Identification of SCs, construction of the expression vector and the effect of two haplotypes on MEG3 transcription. (A) SCs were identified by immunolabeling with anti-Pax7 (red) monolyclonal antibody; Nuclear are counterstained with DAPI (blue); Merged images represented overlays of Pax7 (red) and nuclear staining by DAPI (blue). (B) A FLAG tag sequence (pink) was added at the 3′ end of the MEG3 sequence (gray). This fragment was inserted into the pcDNA3.1(+) vector between the EcoR I and Xho I site. (C) The RT-PCR results of SCs transfected with pcDNA3.1-MEG3-CCCA, pcDNA3.1-MEG3-TTCC and pcDNA 3.1 (+) vector for 48 h. M: Marker 2000, Line 1–3: the RT-PCR results of pcDNA3.1-MEG3-CCCA group, pcDNA3.1-MEG3-TTCC group and pcDNA 3.1(+) group were amplified by MEG3-FLAG, respectively, Line 4: the RT-PCR results of pcDNA 3.1(+) group were amplified by Primer-GAPDH. (D) The effect of two haplotypes on MEG3 transcription. The significance test: ∗∗ P < 0.01.

    Article Snippet: The isolated SCs were seeded in 24-well plates and identified by immunofluorescence staining with anti-mouse Paired box protein Pax7 monoclonal antibody (Santa Cruz Biotechnology, Inc., Europe).

    Techniques: Expressing, Plasmid Preparation, Immunolabeling, Staining, FLAG-tag, Sequencing, Reverse Transcription Polymerase Chain Reaction, Transfection, Marker, Amplification